Cytoplasm Transfer of Nicotiana debneyi to N.tabacum by Protoplast Fusion
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چکیده
منابع مشابه
Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana.
Chloroplasts of Nicotiana tabacum SR1 were transferred into Nicotiana plumbaginifolia by protoplast fusion. The protoplasts of the organelle donor were irradiated with different lethal doses using a (60)Co source, to facilitate the elimination of their nuclei from the fusion products. After fusion induction, clones derived from fusion products and containing streptomycin-resistant N. tabacum SR...
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Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were mapped using two-, three-, and four-factor crosses. The order of the markers was hom-1-thr-1-his-1...
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Objective: To improve the strain of Pleurotus mushroom by protoplast fusion technique Methods: The white rot fungus Pleurotus species was isolated from fresh fruiting body of Oyster mushroom by spore print technique and was cultured on Potato dextrose agar. The selected strain was identified based on the morphological and cultural characters on Potato dextrose agar. The protoplasts were isolate...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1986
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.36.39